Coding

Part:BBa_K2683003

Designed by: Kristi Turton   Group: iGEM18_Lethbridge   (2018-10-07)


MS2 Protein with Polyarginine Peptide

The MS2 protein is a capsid protein originally derived from the MS2 bacteriophage and has been shown to deliver macromolecules in application[1]. A surface modification was added, whereby a polyarginine peptide sequence of 15 Arginines is added to the C-terminus with a thrombin cleavage site in the linker [2]. Polyarginine tags are known to act as a non-specific Cell penetrating peptide[2]. This part was codon optimized for use in E.coli .

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 63
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None